中药/复方具有明显的多靶点特性，对于其复杂的药物机理的阐述，一般需要进行多条信号通路的筛选和排除，以最终锁定关键的一条或多条信号通路。广谱磷酸化抗体芯片技术可同步对30多条信号通路进行广泛筛选，是中药/复方药理研究的常用工具。

|  技术优势

• 高灵敏度/高特异性：采用双抗夹心法，样品不经过变性和前处理，保持天然状态的样品直接检测，对于蛋白的检测准确度高；

• 低样本量：对于信号通路磷酸化检测，抗体芯片只需要100ug总蛋白即可满足实验需求，远低于质谱需要1 mg总蛋白；

• 高效率：芯片上点至的蛋白是已知蛋白，能覆盖大多数经典信号通路，且芯片的数据处理已经成熟化、标准化，得到的数据更聚焦于信号通路本身，花费时间更短。

|  客户案例

客户案例：抗体芯片发现祛湿化瘀汤治疗NASH新机理

在寻找祛湿化瘀汤对于非酒精性脂肪性肝炎(NASH)治疗机理的研究中，研究人员首先通过16S测序，发现药物并未对关键的革兰氏阴性菌产生调控。转而采用PEX100磷酸化抗体芯片，发现了祛湿化瘀汤是通过抑制小肠组织中的MAPK通路,进而抑制细菌際多糖(LPS)对肠道和肝脏的损伤防止NASH的发生。

——  客 户 文 献  ——

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