中文|English
搜索
中文|English
药-靶互作分析 > SPR 表面等离子共振服务
药靶分析封面.png
药靶分析封面.png
  • 药靶分析封面.png
    • 1

SPR 表面等离子共振服务

表面等离子共振SPR技术是一种基于光学物理现象的生物传感分析技术，因其能够实时、无标记地获取分子相互作用的动力学与热力学参数，已成为生物物理与药物研发领域分子互作研究的“金标准”。

表面等离子共振（Surface Plasmon Resonance, SPR）技术是一种基于光学物理现象的生物传感分析技术，因其能够实时、无标记地获取分子相互作用的动力学与热力学参数，已成为生物物理与药物研发领域分子互作研究的“金标准”。

SPR技术原理

表面等离子共振（SPR）是一种发生在金属-介质界面的光学检测技术。当特定角度的入射光照射在金属薄膜表面时，会诱发自由电子产生表面等离子波。若金属表面结合了生物分子，导致局部折射率改变，系统便能通过监测反射光强度的变化（即SPR共振角偏移），实时、动态地定量分析分子间的相互作用。

SPR技术原理示意图（同时作为封面图）.jpg

SPR的独特优势

  • 实时、动态监测分子结合与解离全过程，获得结合速率（ka）、解离速率（kd）及亲和力（KD）等关键动力学参数；

  • 无标记检测，避免标记修饰对分子结构和活性的影响，更真实反映天然互作状态;

  • 高灵敏度检测能力，可捕获弱结合信号的分子互作事件；

  • 兼容多类型样本体系，支持小分子、蛋白、多肽、核酸、多糖及复杂生物样本互作分析

SPR可检测的分子类型

  • 小分子

  • 蛋白质（抗原/抗体/多肽等）

  • 核酸/核酸适配体

  • 脂类/脂质体

  • 多糖、寡糖

  • 纳米粒子

  • 中药复方

SPR的应用领域

基于Biacore体系的SPR检测，能够输出信息丰富、可信度高的结合数据，覆盖靶点结合验证、针对特定靶点的分子库筛选、在中药等复杂样本中垂钓特定靶点的结合分子、分子作用特异性与选择性分析、结合动力学与亲和力测定等多种维度。

达吉特SPR技术平台实力

平台实力：达吉特配备多型号Biacore SPR检测平台，包括Biacore S200、Biacore T200及Biacore 8K等，可覆盖从低通量精细化互作分析到高通量筛选的不同应用需求。同时，平台常备CM5、CM4、CM7、NTA、SA等多种类型传感芯片，可针对不同分子类型、固定策略及实验目的进行灵活选择。

达吉特SPR技术平台配置.jpg

达吉特SPR实测案例赏析

SPR实测赏析1图片1.png

SPR实测赏析1图片2.png



SPR实测赏析1图片3.png


达吉特丰富的SPR项目经验

分子互作研究：达吉特SPR技术平台不仅能够满足常规小分子-蛋白、蛋白-蛋白等分子互作研究需求，还针对复杂生物样本和特殊研究体系开发了多样化检测方案，包括核酸-蛋白互作、纳米材料-蛋白互作、多糖-蛋白互作，以及基于纳米盘稳定表达的多次跨膜蛋白与小分子/蛋白的结合分析等。同时，结合竞争性SPR、动力学参数解析（ka、kd、KD）及结合特异性验证策略，可进一步解析分子间相互作用机制，为靶点验证及作用机制研究提供可靠的数据支持。

截至目前，达吉特已完成SPR相关技术服务项目数百项，累计助力客户发表SCI论文50篇以上，相关研究成果发表于包括Molecular Cancer、Cancer Communications、Gut、Cancer Research、Circulation Research、Nature Communications、Acta Pharmaceutica Sinica B、Advanced Science、Cell Death & Differentiation等国际知名期刊。这些高水平研究成果充分体现了达吉特在药物-靶点互作研究、复杂体系分子结合验证及创新药研发支持领域的技术实力。

客户文献

  1. Ding L, Yin C, Guo Y, et al. Direct coupling and protective activation of DRP1 by the DNA-PKcs inhibitor KU-57788 synergizes with ferroptosis in anaplastic thyroid cancer cells. Cell Death Dis. Published online April 28, 2026. doi:10.1038/s41419-026-08595-3.(浙江省人民医院) IF=9.6

  2. Hou X, Chen L, Huang Y, et al. Natural AhR ligands: A novel approach to dynamic immune regulation in viral infections. Phytomedicine. Published online May 17, 2026. doi:10.1016/j.phymed.2026.158312(南京中医药大学) IF=8.3

  3. Xie LJ, Wei Q, Ni HF, et al. Asiaticoside attenuates renal fibrosis by targeting STAT3 to restore Th17/Treg homeostasis. Phytomedicine. Published online March 11, 2026. doi:10.1016/j.phymed.2026.158049.(东南大学附属中大医院) IF=8.3

  4. Hu M, Xu Y, Wang Y, et al. Gut microbial-derived N-acetylmuramic acid alleviates colorectal cancer via the AKT1 pathway. Gut. Published online February 27, 2025. doi:10.1136/gutjnl-2024-332891(南方医科大学珠江医院) IF=23.1

  5. Xu X, Yan Y, Zheng M, et al. Emodin Alleviates Sepsis-Induced Multiorgan Damage by Inhibiting NETosis through Targeting Neutrophils BCL-10. Adv Sci (Weinh). 2025 Aug 8:e17129.(首都医科大学附属北京中医医院) IF=14.1

  6. Xuan C, Chen D, Zhang S, et al. Isoquercitrin Alleviates Diabetic Nephropathy by Inhibiting STAT3 Phosphorylation and Dimerization. Adv Sci (Weinh). Published online April 4, 2025. doi:10.1002/advs.202414587.(中国人民解放军总医院) IF=14.1

  7. Wang J, Song Y, Tan X, et al. Targeting PIM1 by Bruceine D attenuates skin fibrosis via myofibroblast ferroptosis. Redox Biol. 2025;82:103619. doi:10.1016/j.redox.2025.103619.(第四军医大学西京医院) IF=11.9

  8. Tan J, Feng S, Dang W, et al. Acyl-CoA synthetase long-chain family member 4 (ACSL4)-targeting fluorescent probes for precision intraoperative visualization of hepatocellular carcinoma. J Control Release. Published online September 3, 2025. doi:10.1016/j.jconrel.2025.114161.(复旦大学附属中山医院) IF=11.5

  9. Su T, Shi Z, Tan J, et al. Casticin activates Rheb GTPase by binding to residue Trp141 and induces tumor senescence. Pharmacol Res. 2025;218:107855. doi:10.1016/j.phrs.2025.107855.(广州中医药大学) IF=10.5

  10. Zhao R, Lu Y, Xu Q, et al. Gut blautia coccoides-derived 5Z-dodecenoic acid attenuates chronic psychological stress-induced gastric cancer progression. Int J Surg. Published online November 20, 2025. doi:10.1097/JS9.0000000000004080.(中国人民解放军总医院第一医学中心) IF=10.3

  11. Xia C, Zuo G, Wang M, et al. Targeting HINT1 to improve synaptic plasticity: toward loganin as a new antidepressant strategy. Mol Psychiatry. Published online March 25, 2025. doi:10.1038/s41380-025-02959-5.(中日友好医院) IF=9.6

  12. Chen X, Zhang C, Ye Y, et al. Notopterol attenuates synovitis via α7nAChR-dependent metabolic reprogramming of macrophage polarisation. Phytomedicine. 2026;150:157684. doi:10.1016/j.phymed.2025.157684.(福建中医药大学) IF=8.3

  13. Deng ZH, He LJ, Wei S, et al. Unveiling plumbagin as a novel metabolic modulator to suppress ACC1-mediated de novo lipogenesis in non-small cell lung cancer. Phytomedicine. 2025;148:157438. doi:10.1016/j.phymed.2025.157438.(广西医科大学) IF=8.3

  14. Cheng K, Lin J, Wu M, et al. Berberine promotes hair growth by targeting Axin2 and activating Wnt/β-catenin pathway. Phytomedicine. 2025;141:156669. doi:10.1016/j.phymed.2025.156669.(复旦大学附属华山医院) IF=8.3

  15. Kong Q, Liu S, He S, et al. Celastrol enhanced CD8+T cell immunity in melanoma by targeting SHP2 and upregulating MHC-I. Phytomedicine. 2025;142:156731. doi:10.1016/j.phymed.2025.156731.(复旦大学附属华山医院) IF=8.3

  16. Liao WT, Hu RX, Ji Y, et al. Oleic acid regulates CD4+ T cells differentiation by targeting ODC1-mediated STAT5A phosphorylation in Vogt-Koyanagi-Harada disease. Phytomedicine. 2025;0944-7113. doi:10.1016/j.phymed.2025.156660.(重庆医科大学第一附属医院) IF=8.3

  17. Li Z, Gao K, Wang M, et al. Naringin inhibits the osteoblast-osteoclast pyroptosis cascade reaction mediated by accumulated bone marrow adipose tissue in the treatment of postmenopausal osteoporosis. J Orthop Translat. 2025;55:323-338. Published 2025 Oct 1. doi:10.1016/j.jot.2025.09.004.(山东中医药大学) IF=7.8

  18. Du X, Ma C, Wang Y, et al. The novelty of profilin 2 in regulating pyruvate kinase M2 nuclear translocation and promoting tumor angiogenesis in lung adenocarcinoma. Respir Res. 2025;26(1):203. Published 2025 May 29. doi:10.1186/s12931-025-03281-y.(大连医科大学附属第二医院) IF=5

  19. Peng L, Guo X, Kong X, et al. The mechanism of luteolin suppressing pancreatic cancer (PC) via cyclin B1 (CCNB1)-mediated signalling. Sci Rep. 2025;15(1):42044. Published 2025 Nov 26. doi:10.1038/s41598-025-25973-7.(河南大学第一附属医院) IF=3.9

  20. Zhou J, Song Q, Li H, et al. Targeting circ-0034880-enriched tumor extracellular vesicles to impede SPP1highCD206+ pro-tumor macrophages mediated pre-metastatic niche formation in colorectal cancer liver metastasis. Mol Cancer. 2024;23(1):168. Published 2024 Aug 20. doi:10.1186/s12943-024-02086-9.(上海中医药大学附属曙光医院) IF=27.7

  21. Liu H, Xu L, Yue S, et al. Targeting N4-acetylcytidine suppresses hepatocellular carcinoma progression by repressing eEF2-mediated HMGB2 mRNA translation. Cancer Commun (Lond). 2024;44(9):1018-1041. doi:10.1002/cac2.12595.(华中科技大学同济医学院附属同济医院) IF=24.9

  22. Lin W, Wang N, Wu S, et al. NUAK1-Mediated Phosphorylation of NADK Mitigates ROS Accumulation to Promote Osimertinib Resistance in Non-Small Cell Lung Carcinoma. Cancer Res. 2024;84(23):4081-4098. doi:10.1158/0008-5472.(华中科技大学同济医学院附属协和医院) IF=16.6

  23. Jia L, Jiang Y, Wu L, et al. Porphyromonas gingivalis aggravates colitis via a gut microbiota-linoleic acid metabolism-Th17/Treg cell balance axis. Nat Commun. 2024;15(1):1617. Published 2024 Feb 22. doi:10.1038/s41467-024-45473-y.(首都医科大学附属北京口腔医院) IF=15.7

  24. Li D, Wei R, Zhang X, et al. Gut commensal metabolite rhamnose promotes macrophages phagocytosis by activating SLC12A4 and protects against sepsis in mice. Acta Pharm Sin B. 2024;14(7):3068-3085. doi:10.1016/j.apsb.2024.03.025.(南方医科大学) IF=14.6

  25. Bai M, Xu S, Jiang M, et al. Meis1 Targets Protein Tyrosine Phosphatase Receptor J in Fibroblast to Retard Chronic Kidney Disease Progression. Adv Sci (Weinh). 2024;11(39):e2309754. doi:10.1002/advs.202309754.(南京医科大学附属儿童医院) IF=14.1

  26. Xu L, Liu Q, Liu H, et al. Disrupting CCDC137-mediated LZTS2 and β-TrCP interaction in the nucleus inhibits hepatocellular carcinoma development via β-catenin and AKT. Cell Death Differ. 2025;32(1):134-148. doi:10.1038/s41418-024-01328-z.(华中科技大学) IF=13.7

  27. Liu F, He H, Yang W, et al. Novel energy optimizer, meldonium, rapidly restores acute hypobaric hypoxia-induced brain injury by targeting phosphoglycerate kinase 1. Cell Commun Signal. 2024;22(1):383. Published 2024 Jul 29. doi:10.1186/s12964-024-01757-w.(北京药理毒理研究所) IF=8.9

  28. Zhou W, Zuo H, Qian Y, Miao W, Chen C. Paeoniflorin attenuates particulate matter-induced acute lung injury by inhibiting oxidative stress and NLRP3 inflammasome-mediated pyroptosis through activation of the Nrf2 signaling pathway. Chem Biol Interact. 2024;395:111032. doi:10.1016/j.cbi.2024.111032.(衢州市人民医院) IF=8.3

  29. Wang ZQ, Wu ZX, Chen JW, et al. Cyclovirobuxine D inhibits triple-negative breast cancer via YAP/TAZ suppression and activation of the FOXO3a/PINK1-Parkin pathway-induced mitophagy. Phytomedicine. 2025;136:156287. doi:10.1016/j.phymed.2024.156287.(温州医科大学) IF=8.3

  30. Lu H, Fan L, Zhang W, et al. The mitochondrial genome-encoded peptide MOTS-c interacts with Bcl-2 to alleviate nonalcoholic steatohepatitis progression. Cell Rep. 2024;43(1):113587. doi:10.1016/j.celrep.2023.113587.(西安第四军医大学) IF=7.5

  31. Wang Z, Shao Y, Wu F, et al. Berberine ameliorates vascular dysfunction by downregulating TMAO-endoplasmic reticulum stress pathway via gut microbiota in hypertension. Microbiol Res. 2024;287:127824. doi:10.1016/j.micres.2024.127824.(南方医科大学深圳医院) IF=6.9

  32. Sheng R, Li Y, Wu Y, et al. A pan-PPAR agonist E17241 ameliorates hyperglycemia and diabetic dyslipidemia in KKAy mice via up-regulating ABCA1 in islet, liver, and white adipose tissue. Biomed Pharmacother. 2024;172:116220. doi:10.1016/j.biopha.2024.116220.(北京协和医学院) IF=6.9

  33. Li D, Chang J, Wang Y, et al. Hyperoside mitigates photoreceptor degeneration in part by targeting cGAS and suppressing DNA-induced microglial activation. Acta Neuropathol Commun. 2024;12(1):76. Published 2024 May 16. doi:10.1186/s40478-024-01793-0.(上海中医药大学附属岳阳中西结合医院) IF=5.7

  34. Mei C, Zhang X, Zhi Y, et al. Isorhamnetin Regulates Programmed Death Ligand-1 Expression by Suppressing the EGFR-STAT3 Signaling Pathway in Canine Mammary Tumors. Int J Mol Sci. 2024;25(1):670. Published 2024 Jan 4. doi:10.3390/ijms25010670.(北京市农林科学院畜牧兽医研究所) IF=4.9

  35. Qin Y, Li W, Liu J, et al. Andrographolide ameliorates sepsis-induced acute lung injury by promoting autophagy in alveolar macrophages via the RAGE/PI3K/AKT/mTOR pathway. Int Immunopharmacol. 2024;139:112719. doi:10.1016/j.intimp.2024.112719.(重庆医科大学附属第一医院) IF=4.7

  36. Shan L, Chen Y, An G, et al. Polyphyllin I exerts anti-hepatocellular carcinoma activity by targeting ZBTB16 to activate the PPARγ/RXRα signaling pathway. Chin Med. 2024;19(1):113. Published 2024 Aug 24. doi:10.1186/s13020-024-00984-0.(北京中医药大学) IF=4.7

  37. Luo Z, Li Q, He S, et al. Berberine sensitizes immune checkpoint blockade therapy in melanoma by NQO1 inhibition and ROS activation. Int Immunopharmacol. 2024;142(Pt A):113031. doi:10.1016/j.intimp.2024.113031.(复旦大学附属华山医院) IF=4.7

  38. Wang X, Sui X, Sun Y, et al. Potential Common Mechanisms of Cytotoxicity Induced by Organophosphorus Pesticides via NLRP3 Inflammasome Activation. Geohealth. 2024;8(4):e2023GH000888. Published 2024 Apr 18. doi:10.1029/2023GH000888(东北大学) IF=3.8

  39. Liu Y, Xia G, Chen Y, et al. Purpurolide C-based microneedle promotes macrophage-mediated diabetic wound healing via inhibiting TLR4-MD2 dimerization and MYD88 phosphorylation. Acta Pharm Sin B. 2023;13(12):5060-5073. doi:10.1016/j.apsb.2023.05.032.(首都医科大学口腔医学院) IF=14.6

  40. Li Z, Fan Q, Chen M, et al. The interaction between polyphyllin I and SQLE protein induces hepatotoxicity through SREBP-2/HMGCR/SQLE/LSS pathway. J Pharm Anal. 2023;13(1):39-54. doi:10.1016/j.jpha.2022.11.005.(北京中医药大学) IF=6.1

  41. Yang R, Liu L, Jiang D, et al. Identification of Potential TMPRSS2 Inhibitors for COVID-19 Treatment in Chinese Medicine by Computational Approaches and Surface Plasmon Resonance Technology. J Chem Inf Model. 2023;63(10):3005-3017. doi:10.1021/acs.jcim.2c01643.(深圳市中医院) IF=5.6

  42. Hu X, Li M, Zhang Y, et al. An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1. Exp Cell Res. 2023;424(1):113490. doi:10.1016/j.yexcr.2023.113490.(温州医科大学) IF=3.5

  43. Bai T, Wang X, Qin C, et al. Deficiency of mindin reduces renal injury after ischemia reperfusion. Mol Med. 2022;28(1):152. Published 2022 Dec 12. doi:10.1186/s10020-022-00578-2.(华中科技大学同济医学院附属武汉第一医院) IF=6.4

  44. He S, Wang S, Liu S, Li Z, Liu X, Wu J. Baicalein Potentiated M1 Macrophage Polarization in Cancer Through Targeting PI3Kγ/ NF-κB Signaling. Front Pharmacol. 2021;12:743837. Published 2021 Aug 25. doi:10.3389/fphar.2021.743837.(复旦大学附属华山医院) IF=4.8

  45. Ye S, Luo W, Khan ZA, et al. Celastrol Attenuates Angiotensin II-Induced Cardiac Remodeling by Targeting STAT3 . Circ Res. 2020;126(8):1007-1023. doi:10.1161/CIRCRESAHA.119.315861.(温州医科大学) IF=16.5

  46. Chen X, Zhao Y, Luo W, et al. Celastrol induces ROS-mediated apoptosis via directly targeting peroxiredoxin-2 in gastric cancer cells. Theranostics. 2020;10(22):10290-10308. Published 2020 Aug 15. doi:10.7150/thno.46728.(温州医科大学) IF=12.4




上一个： 没有了
下一个： 药代动力学
返回