Pull down技术是一种有效的筛选药物与潜在靶蛋白之间相互作用的体外技术。利用生物分子之间的亲和力原理，将生物素标记的小分子药物作为“诱饵”固定玉链霉亲和素的磁珠，再从细胞裂解液中“钓取”并富集与之特异性结合的靶点蛋白，随后通过质谱进行药物结合靶蛋白鉴定。

技术优势

1. 基于亲和捕获策略，适用于多类型小分子靶点的富集筛选

2. 在靶细胞/靶组织中进行筛选，靶点的细胞/组织特异性强

3. 高效富集结合蛋白，获得较全面的候选靶点谱

客户案例

案例1：绿原酸靶向RAC1缓解系膜增生性肾小球肾炎

中国人民解放军总医院（301医院）陈香美院士团队在《Acta Pharmaceutica Sinica B》发表题为“Direct pharmacological targeting of RAC1 by chlorogenic acid: A novel therapeutic approach for mesangial proliferative glomerulonephritis”研究成果。研究发现，绿原酸能够直接结合小GTP酶RAC1，抑制RAC1-AKT-THBS1-CD36信号轴活化，从而抑制系膜细胞异常增殖并减轻炎症反应，为MsPGN治疗提供了新的分子机制依据。（达吉特为该研究提供了绿原酸小分子标记和Pull-down+MS靶点筛选服务）

案例2：黄芪来源纳米颗粒通过靶向TLR2增强乳腺癌化疗效果

南京中医药大学团队在《Acta Pharmacologica Sinica B》发表题为“Astragalus-derived nano-agonist potentiates chemotherapy by reducing tumor-suppressive macrophages”的研究论文。本研究首次证实黄芪源外泌体样纳米颗粒（ADNPs）通过其所释放的芒柄花素发挥功效。随后利用生物素标记的芒柄花素进行Pull down筛选，发现其直接靶点TLR2，揭示了ADNPs重编程肿瘤巨噬细胞、增强顺铂化疗疗效，同时缓解骨髓抑制的分子机制。（达吉特为该研究提供了小分子生物素标记与Pull down+MS筛靶服务）

客户文献

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2. Qu Y, Wu L, Wang Y,, et al. Direct pharmacological targeting of RAC1 by chlorogenic acid: A novel therapeutic approach for mesangial proliferative glomerulonephritis, Acta Pharmaceutica Sinica B, https://doi.org/10.1016/ j.apsb.2026.06.041.

3. Han X, Yang YB, Lu QW, et al. Astragalus-derived nano-agonist potentiates chemotherapy by reducing tumor-suppressive macrophages. Acta Pharm Sin B. 2026, ISSN 2211-3835. doi.org/10.1016/j.apsb.2026.03.051.

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